MBS602468 | Synuclein, Nitrated, alpha, beta

Synuclein, Nitrated, alpha, beta | MBS602468 | MyBiosource

Product Short Name: [Synuclein, Nitrated, alpha, beta]

Product Name Synonyme: [Anti -Synuclein, Nitrated, alpha, beta]

Other Names: N/A

Product Gene Name: N/A

Product Gene Name Synonyme: N/A

Other Gene Names: N/A

Clonality: Monoclonal

Isotype: IgG

Clone: [4H317]

Host: Mouse

Reactivity: Human

Specificity: Reacts with the C-terminal region1 of the nitrated/oxidized form of human a-Synuclein, as well as with mutated b-Synuclein.1 The binding of nSyn 12 is dependent upon nitration of either Tyr125 or Tyr136, with the recognition of Tyr136 dominating that of Tyr125.1 On Western blots, it identifies the target band at ~18kD. Reactivity has been confirmed with WT ONOO-, a recombinant wild-type form of a-Synuclein nitrated at Tyr39, 125, 133, and 136 by peroxynitrate, and with human brain extracts by Western blotting. Reactivity has also been confirmed with human DLB (dementia with Lewy bodies) and MSA (multiple system atrophy) brains by immunohistochemistry.1 Cross-reactivity with b-synuclein has been observed using bacterially-expressed and purified recombinant human proteins.1

Purity: Affinity Purified
Highly purified from mouse ascites by protein A affinity.

Form: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

Concentration: N/A

Storage Stability: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Tested Application: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)