MBS430350 | Phosphodiesterase 4C (PDE4C) ACTOne Stable Cell (CHO cell)
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Phosphodiesterase 4C (PDE4C) ACTOne Stable Cell (CHO cell) | MBS430350 | Mybiosource
Product Short Name: [Phosphodiesterase 4C (PDE4C)]
Product Name Synonyme: [Phosphodiesterase 4C (PDE4C) ACTOne Stable Cell Line]
Other Names: [cAMP-specific 3',5'-cyclic phosphodiesterase 4C isoform 1; cAMP-specific 3',5'-cyclic phosphodiesterase 4C; cAMP-specific 3',5'-cyclic phosphodiesterase 4C; phosphodiesterase 4C; DPDE1; PDE21]
Product Gene Name: [PDE4C]
Product Gene Name Synonyme: N/A
Other Gene Names: [PDE4C; PDE4C; DPDE1; PDE21; DPDE1]
Storage Stability: Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130 degree C, preferably in liquid nitrogen vapor, until ready for use.
Low Survival Rate of Cells After Thawing: Cell vials could have thawed accidentally. Store cell vials in liquid nitrogen immediately after receiving and keep frozen at all time.
Leaving the vial at 37 degree C for too long during thawing will lower the survival rate. Place the vial at 37 degree C until cells are just thawed.
Handle the cells gently. Don't tap the vial or pipette the cells too many times before plating the cells.
Replace the medium four hours after thawing or when the cells have settled to remove DMSO.
Slow Growth Rate of Cells: Do not split cells before they have completely recovered from thawing and reach at least 50% confluence.
Do not dilute cells excessively while splitting.
Spit cells before they reach 80 - 90% confluence.
Use Trypsin-EDTA solution to dissociate cells
Cells may not be able to recover to an optimal stage if trypsin- free dissociation buffer is used.
Tested Application: N/A