MBS9719063 | CD20 recombinant protein

MBS9719063 | CD20 recombinant protein | MBS9719063 | Mybiosource

Product Short Name: N/A

Product Name Synonyme:CD80; Activation B7-1 antigen; B7; BB1; CD28LG1; CD28LGB7-1 antigen; T-lymphocyte activation antigen CD80

Other Names: N/A

Product Gene Name: N/A

Product Gene Name Synonyme: N/A

Other Gene Names: N/A

Clonality: N/A

Isotype: N/A

Clone: N/A

Host: HEK293 cells

Reactivity: N/A

Specificity: N/A

Purity:>95% as determined by SDS-PAGE

Form:PBS, pH7.4.

Concentration: N/A

Storage Stability: Store at 2-8 degree C
Expiration: ~12 months

Tested Application: N/A

Background:

 B-lymphocyte antigen CD20 is also known as B-lymphocyte surface antigen B1, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1 and MS4A1, is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+ , CD117+ ) and progressively increasing in concentration until maturity. CD20 is expressed on all stages of B cell development except the first and last; it is present from late pro-B cells through memory cells, but not on either early pro-B cells or plasma blasts and plasma cells. It is found on B-cell lymphomas, hairy cell leukemia, B-cell chronic lymphocytic leukemia, and melanoma cancer stem cells. CD20/MS4A1may be involved in the regulation of B-cell activation and proliferation. Defects in CD20/MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5). CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low.

Sequence:

Amino acid sequence derived from human CD20 (NP_068769.2) (Glu213-Pro297) was expressed with a 6 His tag at the C-terminus.

Protein length:

The recombinant human consists of 91 amino acids and predicts a molecular mass of 10.6 kDa. It migrates as an approximately 23 kDa band in SDS-PAGE under reducing conditions due to glycosylation.

Biological Activity:

Testing in progress. Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Formulation:

Lyophilized from sterile PBS, pH 7.4.