MBS673902 | Mouse Anti-Chicken CD8b-UNLB

Mouse Anti-Chicken CD8b-UNLB | MBS673902 | MyBiosource

Product Short Name: [CD8 beta ]

Product Name Synonyme: [Mouse Anti-Chicken CD8b - Purified, Unlabeled Antibody; Mouse Anti-Chicken CD8 beta ]

Other Names: [CD8 beta; CD8 beta]

Product Gene Name: N/A

Product Gene Name Synonyme: N/A

Other Gene Names: [LOC100136222]

Clonality: Monoclonal

Isotype: Mouse IgG2a kappa

Clone: [EP42]

Host: Mouse

Reactivity: Chicken

Specificity: beta chain (Mr 34 kDa) of chicken CD8
In the chicken, the CD8 molecule is present in two forms: (i) a homodimer of two alpha chains, and (ii) a heterodimer of an alpha chain and a beta chain.1 While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8 alpha - and CD8 beta -chains, a relatively large proportion of the CD8+ TCR- gamma delta cells in the spleens of embryos and young chicks express only the alpha -chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved, but there is a population of TCR- gamma delta CD8 alpha beta cells in the chicken that is not found in rodents.3 Chicken CD8 is expressed on approximately 80% of thymocytes, 45% of blood mononuclear cells and 50% of spleen cells, but <1% of cells in the bursa and bone marrow.1,2 MAb EP42 recognizes the CD8 beta chain.1

Purity: N/A

Form: Purified Antibody

Concentration: N/A

Storage Stability: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.

Tested Application: Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation