MBS673683 | Mouse Anti-Mouse H-2Db-LE/AF

Mouse Anti-Mouse H-2Db-LE/AF | MBS673683 | MyBiosource

Product Short Name: [Mouse Anti-Mouse H-2Db]

Product Name Synonyme: [Mouse Anti-Mouse H-2Db - Low Endotoxin/Azide-Free; Mouse Anti-Mouse H-2Db]

Other Names: N/A

Product Gene Name: N/A

Product Gene Name Synonyme: N/A

Other Gene Names: N/A

Clonality: Monoclonal

Isotype: Mouse (C3H/HeJ) IgG2a kappa

Clone: [28-14-8]

Host: Mouse

Reactivity: Mouse

Specificity: An epitope in the alpha 3 domain of H-2Db
The "classical" MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta 2-microglobulin.3, 4 These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells.5 MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.3, 6 The monoclonal antibody 28-14-8 binds to the alpha 3 domain of H-2Db in the presence or absence of beta 2 microglobulin.7,8 It cross reacts with the alpha 3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq. The antibody has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes,9-11 but it does not block recognition of H-2Ld-positive target cells by Ly- 6G2-positive NK cells.12

Purity: N/A

Form: Low Endotoxin, Azide - Free (LE/AF)

Concentration: N/A

Storage Stability: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

Tested Application: Flow Cytometry; Immunohistochemistry (Frozen Sections)