MBS673269 | Rat Anti-Mouse CD23/FCeRII-LE/AF

Rat Anti-Mouse CD23/FCeRII-LE/AF | MBS673269 | MyBiosource

Product Short Name: [CD23 (Fc epsilon RII)]

Product Name Synonyme: [Rat Anti-Mouse CD23/FCeRII - Low Endotoxin/Azide-Free; Rat Anti-Mouse CD23 (Fc epsilon RII)]

Other Names: N/A

Product Gene Name: N/A

Product Gene Name Synonyme: N/A

Other Gene Names: N/A

Clonality: Monoclonal

Isotype: Rat IgG2a kappa

Clone: [2G8]

Host: Rat

Reactivity: Mouse

Specificity: CD23 low affinity IgE Fc receptor
The CD23 antigen is the low affinity IgE Fc receptor, which is a 49 kDa protein with 38 and 28 kDa fragments.^1 It is expressed on most mature, conventional B cells (but not on peritoneal CD5^+ B cells), and can also be found on the surface of T cells, macrophages and platelets.^1-6 It is distinct from the high affinity IgE receptors found on basophils and mast cells, which mediate allergic reactions. The low affinity receptors are thought to play a role in isotype specific immunoregulation.^3 The regulation of CD23 surface expression appears to be integral with the complex IgE system, which involves interactions of cells, cytokines, antibodies and regulatory factors.^2-6 CD23 has been described as a "membrane bound cytokine," in that the soluble cleavage products of CD23 are themselves able to act as cytokines in vitro.^6

Purity: N/A

Form: Low Endotoxin, Azide - Free (LE/AF)

Concentration: N/A

Storage Stability: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
R-phycoerythrin-Cyanine 7 (R-PE-CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

Tested Application: Flow Cytometry; Immunoprecipitation