MBS673236 | Rat Anti-Mouse CD19-FITC

Rat Anti-Mouse CD19-FITC | MBS673236 | MyBiosource

Product Short Name: [CD19]

Product Name Synonyme: [Rat Anti-Mouse CD19 - FITC; Rat Anti-Mouse CD19]

Other Names: [CD19; B-lymphocyte antigen CD19; B-lymphocyte antigen CD19; OTTHUMP00000122551; differentiation antigen CD19; T-cell surface antigen Leu-12; B-lymphocyte surface antigen B4; CD19 molecule; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12]

Product Gene Name: N/A

Product Gene Name Synonyme: N/A

Other Gene Names: [CD19; CD19; B4; CVID3; MGC12802]

Clonality: Monoclonal

Isotype: Rat (Lewis) IgG2a kappa

Clone: [6D5]

Host: Rat

Reactivity: Mouse

Specificity: CD19 B-cell differentiation antigen, Mr 95 kDa
CD19 is a monomeric transmembrane glycoprotein expressed at relatively constant levels throughout B cell development from early pro-B/pre-B cells (i.e. B220^+/CD43^+/HSA^+) through fully differentiated B cell stages. ^1-7 Terminally differentiated plasma cells do not express CD19. ^1 In humans, the CD19 molecule on the surface of mature B cells associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to provide signal transduction and promote cellular activation. ^3, 4 All splenic and peritoneal IgM^+ cells of both B-1 and B-2 lineages are CD19^+, with B-1 cells expressing higher levels of CD19 than B-2 cells in these sites.1, 7 Recent studies with CD19-deficient mice have suggested that this molecule may not be required for normal generation and maturation of B cells in the bone marrow. ^7 Monoclonal antibody 6D5 recognizes the same epitope as the published clone, 1D3. ^1, 8

Purity: N/A

Form: Fluorescein (FITC) Conjugate

Concentration: N/A

Storage Stability: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or aminecontaining buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC), Spectral Red (SPRD), R-phycoerythrin-Cyanine 7 (R-PE-CY 7) and Rphycoerythrin- Cyanine 5.5 (R-PE-CY 5.5) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The Alexa Fluor 488 (AF488) conjugate is supplied as 0.1mg in 0.2mL of PBS/NaN3. Store at 2-8 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

Tested Application: Flow Cytometry; Immunoprecipitation