MBS673184 | Rat Anti-Mouse CD8a/Ly-2-UNLB

Rat Anti-Mouse CD8a/Ly-2-UNLB | MBS673184 | MyBiosource

Product Short Name: [CD8 alpha /Lyt-2]

Product Name Synonyme: [Rat Anti-Mouse CD8a/Ly-2 - Purified, Unlabeled Antibody; Rat Anti-Mouse CD8 alpha /Lyt-2]

Other Names: N/A

Product Gene Name: N/A

Product Gene Name Synonyme: N/A

Other Gene Names: N/A

Clonality: Monoclonal

Isotype: Rat (LOU/Ws1/M) IgG2a kappa

Clone: [53-6.7]

Host: Rat

Reactivity: Mouse

Specificity: alpha chain of the murine CD8 heterodimer, Mr 32-34 kDa
In the mouse, CD8 exists in two forms: (i) a CD8 heterodimer composed of an alpha chain (CD8 alpha /Lyt-2) and a beta chain (CD8 beta /Lyt-3); and (ii) a homodimer of two alpha chains. The heterodimer is found on the surface of essentially all thymocytes and the "suppressor/cytotoxic" subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8 alpha without CD8 beta. It has been suggested that CD8^+ beta ^- T cells mature extrathymically, while development of the CD8 alpha ^+ beta ^+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8^+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8 alpha ^+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. 1-8

Purity: N/A

Form: Purified (UNLB) Antibody

Concentration: N/A

Storage Stability: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD), R-phycoerythrin-Cyanine 7 (R-PE-CY 7), R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5), allophycocyanin-Cyanine 5.5 conjugate (APC-CY 5.5), allophycocyanin-Cyanine 7 (APC-CY 7) and R-phycoerythrin-Texas Red (R-PE-TXRD) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.

Tested Application: Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation