MBS673129 | Hamster Anti-Mouse CD3e-FITC

Hamster Anti-Mouse CD3e-FITC | MBS673129 | MyBiosource

Product Short Name: [CD3 epsilon ]

Product Name Synonyme: [Hamster Anti-Mouse CD3e - FITC; Hamster Anti-Mouse CD3 epsilon ]

Other Names: [CD3 epsilon; CD3 epsilon]

Product Gene Name: N/A

Product Gene Name Synonyme: N/A

Other Gene Names: [cd3e]

Clonality: Monoclonal

Isotype: Armenian Hamster IgG kappa

Clone: [145-2C11]

Host: Armenian Hamster

Reactivity: Mouse

Specificity: Mouse CD3 epsilon (Mr 25 kDa)
CD3 epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. The 145-2C11 monoclonal antibody recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex. In the presence of Fc receptor-bearing accessory cells, soluble 145-2C11 can activate primed and naïve T cell in vitro. 145-2C11 can also induce redirected lysis of specific target cells by CTL clones and it can block lysis of specific target cells by antigen-specific CTL's. Immobilized 145- 2C11 can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by 145-2C11 may result in apoptotic cell death. ^1-6

Purity: N/A

Form: Fluorescein (FITC) Conjugate

Concentration: N/A

Storage Stability: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) and Cyanine 5 (CY 5) conjugates are supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The bitin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE), allophycocyanin (APC), and Spectral Red (SPRD) conjugates are supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
The Alexa Fluor 647 (AF647) conjugate is supplies as 0.1 mg in 0.2mL of PBS/NaN3. Store at 2-8 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

Tested Application: Flow Cytometry; Immunohistochemistry; Immunoprecipitation; Western Blot