MBS670329 | Rat Anti-Mouse CD3e-CY5

Rat Anti-Mouse CD3e-CY5 | MBS670329 | MyBiosource

Product Short Name: [CD3e-CY5]

Product Name Synonyme: [Rat Anti-Mouse CD3epsilon]

Other Names: N/A

Product Gene Name: N/A

Product Gene Name Synonyme: N/A

Other Gene Names: N/A

Clonality: Monoclonal

Isotype: IgG2b, kappa

Clone: [C363.29B (YCD3-1)]

Host: Rat

Reactivity: Mouse

Specificity: Mouse CD3epsilon (Mr 25 kD)
CD3epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains.1, 2 CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. The monoclonal antibody C363.29B recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex.1 In the presence of Fc receptor-bearing accessory cells, soluble C363.29B can activate primed and naïve T cell in vitro. Immobilized C363.29 monoclonal antibody can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present.1, 3, 4 The monoclonal antibody is cytolytic, easily used for cell surface staining and a good immunoprecipitating antibody.1

Purity: N/A

Form: Cyanine 5 (CY 5) Conjugate

Concentration: N/A

Storage Stability: The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.

Tested Application: Identification and enumeration of CD3+ cells by Flow Cytometry 1, 2, Immunoprecipitation 1, In vitro depletion of CD3+ cells 1, In vitro activation of T cells (accessory cells must be provided) 1, 3, 4 Immunohistochemistry (PS)