MBS438517 | Cyclin B1 (G2- & M-phase Cyclin) Mouse Monoclonal Antibody

Cyclin B1 (G2- & M-phase Cyclin) Mouse Monoclonal Antibody | MBS438517 | MyBiosource

Product Short Name: [Cyclin B1]

Product Name Synonyme: [CCNB, CCNB1, CCNB1_HUMAN, G2 Mitotic Specific Cyclin B1 ]

Other Names: [G2/mitotic-specific cyclin-B1; G2/mitotic-specific cyclin-B1; G2/mitotic-specific cyclin-B1; cyclin B1]

Product Gene Name: [CCNB1]

Product Gene Name Synonyme: N/A

Other Gene Names: [CCNB1; CCNB1; CCNB; CCNB]

Clonality: Monoclonal

Isotype: IgG1, kappa

Clone: [CCNB1/1098 ]

Host: Mouse

Reactivity: Human, Mouse

Specificity: It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.

Purity: N/A

Form: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Concentration: N/A

Storage Stability: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Tested Application: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin