MBS4156805 | Anti-Human IL-1 Receptor Antagonist Antibody

Anti-Human IL-1 Receptor Antagonist Antibody | MBS4156805 | Mybiosource

Product Short Name: [IL-1]

Product Name Synonyme: [Human IL-1 Receptor Antagonist]

Other Names: N/A

Product Gene Name: [IL-1]

Product Gene Name Synonyme: N/A

Other Gene Names: N/A

Clonality: Polyclonal

Isotype: IgG

Clone: N/A

Host: Rabbit

Reactivity: Human

Specificity: This is an IgG preparation of whole rabbit serum purified by DEAE chromatography. In general, this antibody also detects primate IL-1Ra in the same formats using similar dilutions, but not mouse or rabbit IL-1Ra. The antibody does not recognize human IL-1a, IL-1b or IL-1 receptor type I and type II. This antibody is primarily directed against the secreted form of IL-1Ra (also termed sIL-1Ra) but recognizes the native (non-denatured) as well as denatured intracellular form of IL-1Ra (also termed icIL-1Ra). IcIL-1Ra is found in many cells as constitutively expressed protein, particularly epithelial cells and keratinocytes. This antibody does not distinguish between sIL-1Ra and icIL-1Ra in ELISA formats or by immunohistochemistry. When used in immunoblot analysis, it will recognize 17,000 MW IL-1Ra in supernatants released from supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) human serum plus 10 ng/mL E.coli LPS. However, in lysates of PBMC, icIL-1Ra is also found after 48-72 hours of culture. In monocytic cells, the initial IL-1Ra transcript is for sIL-1Ra which can be found almost exclusively intracellularly. In ELISA formats and other immunoreactive assays, this antibody will recognize both sIL-1Ra and icIL-1Ra. Biologically, sIL-1Ra and icIL-1Ra both function as IL-1 receptor antagonists by binding to the type I IL-1R and prevent signaling by IL-1a or IL-1b. However, sIL-1Ra is primarily secreted whereas icIL-1Ra remains associated with the cell. This antibody is also useful for neutralization of human and primate IL-1Ra. If a body fluid, cell supernatant or serum contains IL-1Ra, it is possible to neutralize the inhibitory activity of IL-1Ra on IL-1 activity, being exogenous or endogenous IL-1. A 1:200 dilution of this antibody is incubated with the sample for 4 hours. The mixture is then added to cells in the assay for IL-1 activity. A control of normal rabbit IgG is recommended. An increase in the activity of the sample indicates that the inhibitory activity of IL-1Ra on an IL-1-mediated read-out has been neutralized. Using recombinant human IL-1Ra as a positive control, a 1:200 dilution of this antibody will neutralize the inhibitory activity of IL-1Ra at 100 ng/mL. This antibody does not neutralize the biological activity IL-1. This antibody can be used for FACS analysis. Caution should be exhibited as the F(c) domain of the rabbit IgG molecule may interact with cells non-specifically.

Purity: Affinity Purified

Form: Liquid; PBS, pH 7.4 with 0.05% sodium azide.

Concentration: 1ug/ul

Storage Stability: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Tested Application: ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB), Flow Cytometry (FC/FACS)