MBS298061 | Phospho-STAT3 (Y705) + Total STAT3 TR-FRET Cellular Assay Kit

Phospho-STAT3 (Y705) + Total STAT3 TR-FRET Cellular Assay Kit | MBS298061 | Mybiosource

Product Short Name: [STAT3]

Product Name Synonyme: [Phospho-STAT3 (Y705) and Total STAT3]

Other Names: [signal transducer and activator of transcription 3 isoform 2; Signal transducer and activator of transcription 3; signal transducer and activator of transcription 3; signal transducer and activator of transcription 3; Acute-phase response factor]

Product Gene Name: [STAT3]

Product Gene Name Synonyme: N/A

Other Gene Names: [STAT3; STAT3; APRF; HIES; ADMIO; ADMIO1; APRF]

Clonality: N/A

Isotype: N/A

Clone: N/A

Host: N/A

Reactivity: Human, Mouse, Rat

Specificity: HeLa cells were seeded at 40,000 cells/well and cultured overnight at 37 degree C, 5% CO2, in DMEM with 10% FBS. Cells were treated with either IFN-alpha for 30 min (stimulation) or with JAK inhibitor 1for 30 min then 1.5 nM IFN-alpha (inhibition). The media was removed from the wells, and cells were lysed with 50 uL/well of 1X Lysis Buffer #2 containing 1 mM NaF and 2 mM Na3VO4, on an orbital shaker (400 rpm) for 30 min at room temperature. The lysates were then analyzed for total-STAT3 using the two-plate assay protocol. Lysates analyzed on separate wells for phospho-STAT3 (Y705) are also shown for comparison. The TR-FRET signal was recorded at 665 and 615 nm (EnVision lamp excitation) after a 4 h incubation period at room temperature.

Purity: N/A

Form: N/A

Concentration: N/A

Storage Stability: Store at -80 degree C.

Tested Application: Cell-Based Assays