MBS298040 | Phospho-NF-kappaB (S536) + Total NF-kappaB TR-FRET Cellular Assay Kit

Phospho-NF-kappaB (S536) + Total NF-kappaB TR-FRET Cellular Assay Kit | MBS298040 | Mybiosource

Product Short Name: [NF-kappaB]

Product Name Synonyme: [Phospho-NF-kappaB (S536) and Total NF-kappaB]

Other Names: [transcription factor p65 isoform 2; Transcription factor p65; transcription factor p65; RELA proto-oncogene, NF-kB subunit; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3]

Product Gene Name: [NF-kappaB]

Product Gene Name Synonyme: N/A

Other Gene Names: [RELA; RELA; p65; CMCU; NFKB3; NFKB3]

Clonality: N/A

Isotype: N/A

Clone: N/A

Host: N/A

Reactivity: Human

Specificity: HeLa cells were seeded at 40,000 cells/well and cultured overnight at 37 degree C, 5% CO2, in DMEM with 10% FBS. Cells were then treated with TNF-alpha for 10 min at room temperature. The media was removed from the wells, and cells were lysed with 50 uL/well of 1X Lysis Buffer #2 containing 1 mM NaF and 2 mM Na3VO4, on an orbital shaker (400 rpm) for 30 min at room temperature. The lysates were then analyzed for total-NF-kappaB using the two-plate assay protocol. Lysates analyzed on separate wells for phospho-NF-kappaB (S536) are also shown for comparison. The TR-FRET signal was recorded at 665 and 615 nm (EnVision lamp excitation) after a 4 h incubation period at room temperature.

Purity: N/A

Form: N/A

Concentration: N/A

Storage Stability: Store at -80 degree C.

Tested Application: Cell-Based Assays