MBS673976 | Mouse Anti-Chicken CD8a-BIOT

Mouse Anti-Chicken CD8a-BIOT | MBS673976 | MyBiosource

Product Short Name: [CD8 alpha ]

Product Name Synonyme: [Mouse Anti-Chicken CD8a - Biotin; Mouse Anti-Chicken CD8 alpha ]

Other Names: [CD8 antigen, alpha polypeptide; CD8 antigen, alpha polypeptide; CD8 antigen, alpha polypeptide; CD8 alpha; CD8 antigen, alpha polypeptide]

Product Gene Name: N/A

Product Gene Name Synonyme: N/A

Other Gene Names: [cd8a; cd8a; MGC136643; zgc:136643]

Clonality: Monoclonal

Isotype: IgG2b kappa

Clone: [3-298]

Host: Mouse

Reactivity: Chicken

Specificity: alpha chain of chicken CD8 (Mr 34 kDa)
In the chicken, the CD8 molecule is present in two forms: (i) a homodimer of two alpha chains, and (ii) a heterodimer of an alpha chain and a beta chain.1,2 Chicken CD8 is expressed on approximately 80% of thymocytes, 45% of blood mononuclear cells and 50% of spleen cells, but <1% of cells in the bursa and bone marrow.1 While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8 alpha - and CD8 beta -chains, a relatively large proportion of the CD8+ TCR- gamma delta cells in the spleens of embryos and young chicks express only the alpha -chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved, but there is a population of TCR- gamma delta CD8 alpha beta cells in the chicken that is not found in rodents.3 MAb 3-298 recognizes the CD8 alpha chain of both the chicken and turkey.4

Purity: N/A

Form: Biotin (BIOT) Conjugate

Concentration: N/A

Storage Stability: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.

Tested Application: Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation