MBS602250 | Muscarinic Acetylcholine Receptor M2, aa225-359 (Chrm2, CHRM-2, 7TM Receptor, FLJ43243, HM2, MGC120006, MGC120007)

Muscarinic Acetylcholine Receptor M2, aa225-359 (Chrm2, CHRM-2, 7TM Receptor, FLJ43243, HM2, MGC120006, MGC120007) | MBS602250 | MyBiosource

Product Short Name: [Muscarinic Acetylcholine Receptor M2, aa225-359]

Product Name Synonyme: [Anti -Muscarinic Acetylcholine Receptor M2, aa225-359 (Chrm2, CHRM-2, 7TM Receptor, FLJ43243, HM2, MGC120006, MGC120007)]

Other Names: [muscarinic acetylcholine receptor M2; Muscarinic acetylcholine receptor M2; muscarinic acetylcholine receptor M2; 7TM receptor; OTTHUMP00000208393; OTTHUMP00000208394; OTTHUMP00000208395; OTTHUMP00000208396; muscarinic M2 receptor; cholinergic receptor, muscarinic 2, isoform a; cholinergic receptor, muscarinic 2]

Product Gene Name: N/A

Product Gene Name Synonyme: N/A

Other Gene Names: [CHRM2; CHRM2; HM2; FLJ43243; MGC120006; MGC120007]

Clonality: Monoclonal

Isotype: IgG2a

Clone: [2Q147]

Host: Rat

Reactivity: Human, Monkey, Rat

Specificity: M2 muscarinic acetylcholine receptor. No reactivity with the other subtypes. Species crossreactivity: Human, monkey and rat. Expected to react with most mammalian species.

Purity: Purified
Purified immunoglobulin

Form: Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

Concentration: N/A

Storage Stability: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

IMMUNOHISTOCHEMISTRY PROTOCOL
Use on 30 um, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation.
1. 3 x 10 minute washes in TBS (with or without 0.25% Triton).
2. Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).
3. Incubate M9520-13B diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at RT followed by 16 hours at 4 degree C.
4. 3 x 10 minute washes in TBS.
5. Incubate with secondary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody).
6. 3 x 10 minute washes in TBS.
7. ABC Elite (1:200) in TBS.
8. 2 x 10 minute washes in TBS.
9. 1 x 10 minute wash in phosphate buffer (no saline).
10. DAB reaction with 0.06% NiCl added for intensification.
11. 2 x 10 minute washes in PBS.
12. 1 x 10 minute wash in phosphate buffer (no saline).

Tested Application: Immunohistochemistry (IHC), Immunocytochemistry (ICC)