MBS570349 | Mouse anti CD3

Mouse anti CD3 | MBS570349 | MyBiosource

Product Short Name: [CD3]

Product Name Synonyme: N/A

Other Names: [cd3; CD3gamma/delta; CD3gamma/delta]

Product Gene Name: [CD3]

Product Gene Name Synonyme: N/A

Other Gene Names: [cd3g]

Clonality: N/A

Isotype: IgG1

Clone: [UCHT1]

Host: Mouse

Reactivity: Human

Specificity: The CD3 mAb (clone UCHT1) recognizes cytoplasmic CD3 epsilon in precursor T-cells and cytoplasmic and surface CD3 epsilon in mature T-lymphocytes.

The sensitivity of UCHT1 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity).The sensitivity of UCHT1 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity).In practice, 50 ul of leukocytes containing 107 cells/ml are stained with 20 ul mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold. In addition and to control lot-to-lot variation, the given lot is compared and adjusted to fluorescence standards with defined intensity

Purity: N/A

Form: Purified by Chromatography, Storage buffer: PBS pH 7.2, 1% BSA, 0.05% NaN3

Concentration: N/A

Storage Stability: Monoclonal antibody reagents contain optimal concentrations of affinity-purified antibody. For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8 degree C (DO NOT FREEZE!) and protected from prolonged exposure to light. Stability of the reagent: Please refer to the expiry date printed onto the vial. The use of the reagent after the expiration date is not recommended.

Tested Application: Flow Cytometry (FC/FACS), Indirect Immunofluorescence (IF)